Purpose

Staphylococcus aureus (SA) is a leading cause of morbidity mortality and as a result it is responsible for a marked economic burden on today"s health care system. Infections with SA that are resistant to Methicillin antibiotic (MRSA) are very common and are a major part of this burden. 30% of the population are colonized with S. aureus so at least theoretically their immune system is constantly exposed to the bacteria and expected to induce protective immunity. However at the same time 30% of the population experiencing an infection with S. aureus will be afflicted with recurrent infections with this bacterium and despite previous exposure. Developing a vaccine would be an ideal solution for prevention of these infections. However despite intensive study in the last 3 decades a successful vaccine against S.aureus is still unavailable. For developing and designing an efficient vaccine a better understanding of the immune response to S. aureus is needed..For many yearsthe concept was that B cell responses and antibody production are enough for protection against S.aureus. In recent years direct evidence from KO mice as well as evidence from humans with primary immune deficiency demonstrates that CD4 Th1 and Th17 immune responses are necessary for protection from S. aureus. Nevertheless our recent results demonstrate that the Th1 and Th17 response to the various S. aureus strains varied extensively in response to in-vitro stimulation. Further wide variability was also inspected in IgG expression by proliferating B cells in response to the different strains. We could show that mobile genetic elements carried by phages are responsible for a significant portion of this immune response variability. These findings are bringing up the possibility that the strain specificity is a new factor that might need to be taken into account when we are evaluating protection. At this time point what is not known/understood and what we want to clarify in the current study is: (i) Whether previous exposure to various strains results in different level of protective memory. (ii) How immune memory induced by exposure to one strain affect the ability to respond to other strains of S. aureus.

Condition

Eligibility

Eligible Ages
Between 18 and 60
Eligible Genders
All
Accepts Healthy Volunteers
Yes

Inclusion Criteria

  • Healthy volunteer 18 - 60 years of age
  • Willing to refrain from using deodorant on the day of the study visit.
  • Willing to refrain from showering for a 10 hr period prior to swabbing

Exclusion Criteria

  • HIV positive
  • Immunosupressed or taking any immunosuppresive medication
  • History of active hepatitis B or C
  • congenital immunodeficiency
  • History of nasal surgery
  • Ingestion of antibiotics within the last 30 days
  • Any history of receiving chemotherapy or radiation
  • Any history of atopic dermatitis or psoriasis
  • Currently enrolled in an interventional research study
  • Any medical psychological or social condition that in the opinion of the Investigator would jeopardize the health or well-being of the participant during any study procedures or the integrity of the data.
  • Received any vaccine within one month of the second visit
  • Steroid treatment by mouth skin (topical) or inhalation

Study Design

Phase
Study Type
Observational

More Details

Status
Recruiting
Sponsor
Rockefeller University

Study Contact

Recruitment Office
8007822737
rucares@rockefeller.edu

Notice

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