The Rockefeller University

Investigating the Role of Neutrophil Extracellular Traps (NETs) in Fibrolamellar Carcinoma

Purpose

data-pm-slice=0 0 []>Fibrolamellar carcinoma (FLC) is a rare liver cancer that primarily affects adolescents and young adults with no prior liver disease. It is defined by a unique genetic fusion and patients often present with vague symptoms which can delay diagnosis and limit treatment options. Currently there are no reliable blood tests or biomarkers to detect or monitor FLC making it difficult to track disease progression or response to therapy.Neutrophils are a type of white blood cell that help defend against infection. When activated neutrophils can release web-like structures called neutrophil extracellular traps (NETs). While NETs play a role in fighting infections research in other cancers suggests they may also promote tumor growth spread and resistance to therapy. However the role of NETs in FLC remains unknown.This study will specifically examine whether neutrophils from FLC patients are capable of forming NETs in the laboratory (in vitro). Patients with FLC will provide a blood sample and neutrophils will be isolated and tested under controlled conditions to measure NET release using imaging and biochemical methods. To provide a comparison a group of healthy volunteers will also donate blood samples so that differences in NET formation between FLC patients and healthy controls can be evaluated.The knowledge gained from this project may provide important insight into how the immune system interacts with FLC and may eventually guide the development of new biomarkers or therapeutic approaches for this rare cancer.

Condition

• Fibrolamellar hepatocellular carcinoma